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Bioluminescence determination of caspase-3/7 in single osteosarcoma cells
Killinger, Michael ; Matalová, Eva ; Klepárník, Karel
The protein heterogeneity at the single-cell level has been recognized to be vital for an understanding of various life processes during animal development. In addition, the knowledge of accurate quantity of relevant proteins at cellular level is essential for appropriate interpretation of diagnostic and therapeutic results. Here, we determined the level of active caspase-3/7 in physiological and pathological (cancer) conditions by our miniaturized instrumentation.

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